A STUDY OF URICASE BIOSENSOR BASED ON A GLASSY-CARBON ELECTRODE MODIFIED WITH NAFION AND METHYL VIOLOGEN

Authors
JIN LT YE JS TONG W FANG YZ
Citation
Lt. Jin et al., A STUDY OF URICASE BIOSENSOR BASED ON A GLASSY-CARBON ELECTRODE MODIFIED WITH NAFION AND METHYL VIOLOGEN, Mikrochimica acta, 112(1-4), 1993, pp. 71-75
Citations number
7
Categorie Soggetti
Chemistry Analytical
Journal title
Mikrochimica acta → ACNP
ISSN journal
00263672
Volume
112
Issue
1-4
Year of publication
1993
Pages
71 - 75
Database
ISI
SICI code
0026-3672(1993)112:1-4<71:ASOUBB>2.0.ZU;2-T
Abstract
A uricase biosensor was constructed by using bovine serum albumin and glutaraldehyde as cross linker to immobilize uricase on a glassy carbo n electrode modified with Nafion and methyl viologen (MV). A linear re sponse of uric acid in serum was observed in a range of 1.0 . 10(-3) t o 5.0 . 10(-6) mol/l and the response time is 25 s. This biosensor has the advantage of high sensitivity, fast response as compared with pre vious sensors and low interferences.