The influences of initial sodium chloride (6% and 0% w/v in tap water)
and acetic acid concentrations (0.3%, and 0.6% v/v), use of starter c
ulture, and aerobic versus anaerobic conditions on the biochemical cha
nges that take place throughout the preservation stage of ripe olive p
rocessing were investigated. Glucose, fructose and sucrose were comple
tely consumed during preservation. Mannitol and malic acid were metabo
lized only in the presence of lactic acid bacteria or oxidative yeast
(aerobic treatment). The main metabolites produced were lactic and ace
tic acid in aerobic or anaerobic treatments inoculated with Lactobacil
lus plantarum. Methanol and ethanol were present in all the brines alt
hough in a lower concentration when conditions were aerobic. Thus, ind
uced lactic fermentation led to the most efficient utilization of carb
ohydrates and yielded the most suitable physicochemical characteristic
s for ripe olive preservation.