Decontamination of plastic and wooden cutting boards was studied, with
a view to preventing cross-contamination of foods in home kitchens. N
ew and used plastic (four polymers plus hard rubber) and wood (nine ha
rdwoods) boards were cut into 5-cm square blocks (25 cm(2) area) for t
hese experiments. Bacterial contaminants--Escherichia coli (two nonpat
hogenic strains plus serotype 0157:H7), Listeria innocua, L. monocytog
enes, or Salmonella typhimurium--applied to the block surface in nutri
ent broth or chicken juice, were recovered by soaking the surface in n
utrient broth or pressing the block onto nutrient agar, within minutes
or greater than or equal to 12 h later. Persistence and overnight mul
tiplication of bacteria on plastic surfaces depended on maintenance of
humidity so as to prevent drying of the contaminant. New plastic cutt
ing surfaces were relatively easy to clean and were microbiologically
neutral, but plastic boards with extensive knife scars were difficult
to clean manually, especially if they had deposits of chicken fat on t
hem. Fewer bacteria were generally recovered from wooden blocks than f
rom plastic blocks. Clean wood blocks rapidly absorbed all of the inoc
ulum, after which the bacteria could not be recovered within 3 to 10 m
in. If the board surface was coated with chicken fat, some bacteria mi
ght be recovered even after 12 h at room temperature and high humidity
. Cleaning with hot water and detergent generally removed these bacter
ia, regardless of bacterial species, wood species, and whether the woo
d was new or used.