The biogenesis of ''fusty'' defect was studied by chemical and microbi
al analyses on olives stored in piles for different times and their re
sulting oils. The fusty defect was perceived by tasters after four day
s of storage. The quali-quantitative composition of oil volatile fract
ion was a very suitable way to emphasize metabolites produced by micro
organisms involved during the fruit storage. Some volatile compounds,
such as 2- and 3- methyl butan-1-al, their corresponding alcohols and
propionic acid, 2-methyl propionic acid and 3-methyl butanoic acid, we
re produced. At he same time, it was observed a dramatic development o
f Clostridium sp. and, in a lower proportion, of Pseudomonas sp. Furth
ermore, during the storage the evident softening of fruits has to be a
ttributed to microorganisms in rapid growth belonging to Enterobacter
so. and moulds, that have in its enzymatic store pectinoltic enzymes.