DETECTION OF SALMONELLA-ENTERITIDIS IN FECES FROM POULTRY USING BOOSTER POLYMERASE CHAIN-REACTION AND OLIGONUCLEOTIDE PRIMERS SPECIFIC FOR ALL MEMBERS OF THE GENUS SALMONELLA
Nd. Cohen et al., DETECTION OF SALMONELLA-ENTERITIDIS IN FECES FROM POULTRY USING BOOSTER POLYMERASE CHAIN-REACTION AND OLIGONUCLEOTIDE PRIMERS SPECIFIC FOR ALL MEMBERS OF THE GENUS SALMONELLA, Poultry science, 73(2), 1994, pp. 354-357
Salmonella enteritidis was identified in feces from hens using the pol
ymerase chain reaction (PCR) and oligonucleotide primers specific for
all members of the genus Salmonella. Feces from specific-pathogen-free
Leghorn hens were determined to be negative for Salmonella by microbi
ological culture and by the PCR. Fecal samples were inoculated with kn
own numbers of colony-forming units of S. enteritidis. The DNA was ext
racted from fecal samples and amplified by the PCR using genus-specifi
c primers. Salmonella were detected in all samples known to be positiv
e; the sensitivity of the assay extended to 1 cfu of S. enteritidis/g
feces. Feces that were not inoculated with Salmonella were negative. M
icrobiological culture was less sensitive than the PCR assay; results
of culture of feces with less than 10(2) cfu/g were negative. Although
S. enteritidis was used in this study, the oligonucleotide primers us
ed in this study have been previously demonstrated to be genus-specifi
c for Salmonella.