O. Meyer et al., CARCINOGENICITY STUDY OF THE EMULSIFIER TOSOM AND THE RELEASE AGENT TOS IN WISTAR RATS, Food and chemical toxicology, 31(11), 1993, pp. 825-833
Groups of 60 Wistar rats of each sex were fed diets containing 3, 6 or
12% of the margarine emulsifier TOSOM (thermally oxidized soybean oil
interacted with mono- and diglycerides of fatty acids) for 2.5 yr. In
addition, three groups of 60 rats of each sex were fed two products o
f the release agent TOS (thermally oxidized soybean oil) in dietary le
vels of 1.2% TOS(G) (TOS from Grindsted Product A/S, Denmark) and 0.3
and 1.2% TOS(N) (TOS from Nexus Aps, Denmark), respectively for 2.5 yr
. 120 rats of each sex fed a diet containing mono- and diglycerides se
rved as controls. The diets given to all groups were isocaloric. Clini
cal appearance, food consumption, body weight and weight gain, surviva
l, haematology, and clinical chemistry parameters were examined. Gross
and histopathological examinations, including neoplastic and non-neop
lastic lesions, were performed on all groups. Time to occurrence of tu
mours was recorded. No substance-related effect, including carcinogeni
city, was found.