BIOCHEMICAL-STUDIES OF SOME NONCONVENTIONAL SOURCES OF PROTEINS .6. PHYSICOCHEMICAL PROPERTIES OF APRICOT KERNEL PROTEINS AND THEIR CHANGESDURING DETOXIFICATION

Authors
RAHMA EH ELADAWY TA LASZTITY R GOMAA MA ELBADAWEY AA GAUGECZ J
Citation
Eh. Rahma et al., BIOCHEMICAL-STUDIES OF SOME NONCONVENTIONAL SOURCES OF PROTEINS .6. PHYSICOCHEMICAL PROPERTIES OF APRICOT KERNEL PROTEINS AND THEIR CHANGESDURING DETOXIFICATION, Die Nahrung, 38(1), 1994, pp. 3-11
Citations number
21
Categorie Soggetti
Food Science & Tenology
Journal title
Die Nahrung → ACNP
ISSN journal
0027769X
Volume
38
Issue
1
Year of publication
1994
Pages
3 - 11
Database
ISI
SICI code
0027-769X(1994)38:1<3:BOSNSO>2.0.ZU;2-C
Abstract
The physico-chemical properties of apricot kernel proteins before and after detoxification treatments with water and ammonium hydroxide were determined. Water treament shifted the lambda(max) to 278 nm while am monium hydroxide has no effect. Fluorescence spectrum showed emission red shift and quenching for both detoxification treatments. Sedimentat ion velocity pattern of the untreated proteins contained two peaks hav ing S20,w values of 6.8S and 11.1S. Detoxified kernels showed aggregat es of 15.4S and 16.9S due to water and ammonium hydroxide treatment, r espectively. Gel filtration pattern consisted of three peaks for all s amples, meanwhile ion-exchange chromatography showed an additional pea k. HPLC, SDS-PAGE and isoelectric focusing-PAGE proved dissociation of the high molecular weight proteins as well as removal of the low mole cular weight protein fractions due to detoxification treatments.