A TECHNIQUE FOR CANNULATING THE CISTERNA-MAGNA AND SAMPLING CEREBROSPINAL-FLUID FROM SOCIALLY HOUSED BIRDS

The measurement of central levels of neurochemicals is an important ap proach to the understanding of the neurophysiological basis of behavio r patterns in animals. Previous studies have utilized central sampling techniques developed for individually housed animals. The purpose of this study was to develop a cannulation technique and a method for sam pling cerebrospinal fluid (CSF) from socially housed birds to facilita te the study of the neurophysiological basis of social behaviors. The cannulation technique involved the surgical implantation of a 22-gauge concentric guide cannula into the cisterna magna of 16-wk-old, feed-r estricted male broiler breeders (n = 6). Individual-specific coordinat es and optimum angle and depth of implantation of the cannula were det ermined in order to place the cannula correctly in the designated site . Once implanted, the guide cannula proved to be unobtrusive and secur e and did not attract aggressive pecking from other birds in the pen. Two methods of CSF sampling were then examined. The first method requi red the use of a push-pull perfusion pump to withdraw CSF at a rate of 1 to 2 muL/min. The second method (passive), which did not use a pump , involved simply removing a ''dummy'' cannula from the guide cannula to release the CSF, which was then collected with a glass Hamilton syr inge. Samples ranging from 100 to 500 muL were collected using the pas sive method. The combination of the cannulation technique described an d the passive sampling method proved to be the most simple, efficient, and reliable method for measuring central levels of neurochemicals in socially housed broiler breeder males.