SIMULTANEOUS DETERMINATION OF RESIDUAL SY NTHETIC ANTIBACTERIALS IN FISH AND MEAT-PRODUCTS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH PHOTODIODE-ARRAY DETECTOR

A procedure was developed for simultaneous determination of 21 synthet ic antibacterials in fish and meat products by high performance liquid chromatography with a photodiode array detector. The antibacterials w ere extracted with acetonitrile-0.2% metaphosphoric acid (7:3), follow ed by liquid-liquid partition with dichloromethane. High performance l iquid chromatography was carried out on a TSK-gel ODS-80Ts column usin g gradient elution with acetonitrile-sodium dihydrogenphosphate. The r ecoveries of antibacterials from fish and meat products spiked at 1.0 mug/g were over 70% except in the cases of sulfisomidine and carbadox. The detection limits were 0.05-0.10 mug/g for each antibacterial exce pt thiamphenicol (0.25 mug/g).