SYNTHESIS OF PHOSPHATIDYLGLYCEROL FROM SOYBEAN LECITHIN WITH IMMOBILIZED PHOSPHOLIPASE-D

Phosphatidylglycerol (PG) was synthesized from soybean lecithin with t he help of phospholipase D (PLD) that was immobilized with calcium alg inate gel-enveloped polyethyleneimine-glutarald ehyde. The immobilizat ion conditions were optimized with response surface analysis: Ca-algin ate 1.39%, polyethyleneimine 7.78%, glutaraldehyde 1.22%. The conversi on of phosphatidylglycerol was 87%. The properties of the immobilized PLD were: optimal temperature 25-30 degrees C, optimal pH 8.2, ratio o f ether phase and water phase 1.5-2.5:1 (vol/vol), repeated 15 batches operating with stabilizing conversion percentage of phosphatidylglyce rol.