BACTERIAL ADHERENCE AND VIABILITY ON CUTTING BOARD SURFACES

The adherence and viability of Escherichia coli inoculated onto the su rfaces of plastic cutting boards and new and used wood cutting boards were evaluated. Most of the inoculum was recovered from all surfaces a fter resident drying times of 5 min and from plastic surfaces at 24 h. When the exposure time was extended to 2 h, > 90% of the cells placed on new and used dry wood surfaces were not recovered after vigorous r insing. Scanning electron microscopy showed that the bacteria resided within the structural xylem fibers and vegetative elements of the wood . After resident drying times of up to 2 h, almost 75 % of the adheren t bacteria on the wood surfaces were viable, as defined by a nalidixic acid direct viable count procedure. Microcosm studies showed no intri nsic growth-supporting or toxic properties of the cutting board materi als. Bacteria that adhered to plastic surfaces were more easily remove d by low-temperature washing than were cells that adhered to wood surf aces. These studies demonstrated that bacteria adhering to wood surfac es resided within the structural and vegetative elements of the wood's xylem tissues and were viable; wood was more retentive than plastic; penetration of the inoculum liquid promoted cell adherence to the wood matrix; and conditioning of wood with water before inoculation interf ered with bacterial adherence.