SIMPLE EXTRACTION OF CAMPYLOBACTER LIPOPOLYSACCHARIDE AND PROTEIN ANTIGENS AND PRODUCTION OF THEIR ANTIBODIES IN EGG-YOLK

Authors
CHANDAN V FRASER ADE BROOKS BW YAMAZAKI H
Citation
V. Chandan et al., SIMPLE EXTRACTION OF CAMPYLOBACTER LIPOPOLYSACCHARIDE AND PROTEIN ANTIGENS AND PRODUCTION OF THEIR ANTIBODIES IN EGG-YOLK, International journal of food microbiology, 22(2-3), 1994, pp. 189-200
Citations number
15
Categorie Soggetti
Food Science & Tenology",Microbiology
Journal title
International journal of food microbiology → ACNP
ISSN journal
01681605
Volume
22
Issue
2-3
Year of publication
1994
Pages
189 - 200
Database
ISI
SICI code
0168-1605(1994)22:2-3<189:SEOCLA>2.0.ZU;2-3
Abstract
Antigens were heat extracted from Campylobacter jejuni (LI04) and C co li (LI020) in the presence of ethylenediaminetetraacetate (EDTA) and w ere recovered in the supernatant of a low-speed centrifugation. The me thod is simpler, safer and more efficient in extracting lipopolysaccha ride (LPS) antigens than the hot phenol method. The extracted antigens (LPS plus several proteins) elicited production of antigen-specific a ntibodies in the egg yolk of immunized hens. Antibodies purified by po lyethyleneglycol fractionation were used to detect antigens fractionat ed on SDS polyacrylamide gel electrophoresis.