HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC A NALYSIS OF SULFONAMIDES IN LIVESTOCK PRODUCTS USING MATRIX SOLID-PHASE DISPERSION (MSPD) METHOD WITH SILICA-GEL
H. Tamura et al., HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC A NALYSIS OF SULFONAMIDES IN LIVESTOCK PRODUCTS USING MATRIX SOLID-PHASE DISPERSION (MSPD) METHOD WITH SILICA-GEL, Shokuhin Eiseigaku Zasshi, 35(3), 1994, pp. 271-275
The MSPD method with silica gel (Silica Gel-MSPD) was developed for th
e extraction of sulfonamides in livestock products. Residual sulfonami
des such as sulfameradine (SMR). sulfadimidine (SDD), sulfamonomethoxi
ne (SMM), sulfadimethoxine (SDM) and sulfaquinoxarine (SQO) were extra
cted from chicken muscle tissue, yolk and pork muscle tissue. A sample
(0.5 g) was mixed with 0.7 g of silica gel and 1.5 ml of acetonitrile
, and the mixture was dried, washed with hexane and eluted with methan
ol or tetrahydrofuran (THF). An aliquot of the methanol or THF solutio
n was subjected to HPLC for the determination of each sulfonamide. The
recoveries of sulfonamides added to the chicken, yolk and pork were 7
8.1 to 93.1%, 74.6 to 86.2% and 69.6 to 87.1%, respectively. The limit
s of detection for the proposed method were 0.01 ppm for SMR and SDD,
0.02 ppm for SMM and 0.04 ppm for SDM and SQO. In order to confirm the
utility of the proposed method, analyses of commercial products were
performed, and the result was compared with that obtained by MSPD with
ODS. Consequently, 0.94 ppm of SMM and 0.99 ppm of SDM were detected
in two samples of pork muscle by the proposed method, whereas the valu
es detected by using ODS were 0.81 and 0.56 ppm, respectively.