PURIFICATION AND CHARACTERIZATION OF GLYCEROL PHOSPHATE DEHYDROGENASEPRODUCED FROM WHEAT BRAN USING A MIXED CULTURE SYSTEM

Glycerol phosphate dehydrogenase was produced from wheat bran by the m ixed culture fermentation technique. The process involved saccharifica tion of cellulose to glucose by Trichoderma viride followed by convers ion of glucose into glycerol by Saccharomyces cerevisiae Y-1347. Diffe rent purification steps were applied to the culture filtrate to obtain a pure enzyme preparation. The pure preparation indicated that the mo lecule consists of one peptide chain, with a molecular weight of 51 00 0 and isoelectric point of 5.7. The amino acid content was also studie d. Lineweaver-Burk analysis gave a k(m) value of 0.033 mmol and V-max of 83.3 mmol/ml/min. The enzyme showed its maximum activity at pH 6.0 when incubated at 25 degrees C for 10 min.