We. Neff et al., SILVER ION HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF THE TRIACYLGLYCEROLS OF CREPIS-ALPINA SEED OIL, Journal of the American Oil Chemists' Society, 71(8), 1994, pp. 853-855
The triacylglycerols of Crepis alpina oil were characterized because t
his oil has a high concentration of crepenynic (cis-9-odadecen-12-ynoi
c) acid, a fatty acid useful in the chemical synthesis of deuterated f
ats for human metabolism studies. The triacylglycerols were separated
from the crude oil by solid-phase extraction. Resolution, quantitation
and isolation of the individual triacylglycerols were performed by si
lver ion high-performance liquid chromatography on a commercial column
, an acetonitrile in hexane isocratic mobile phase and flame-ionizatio
n detection. Isolated triacylglycerols were identified by capillary ga
s chromatography of their fatty acid methyl esters. Of the eleven elut
ed triacylglycerols of Crepis alpina oil, 85% included 35% tricrepenyn
oyl, 34% linoleoyldicrepenynoyl and 16% dilinoleoylcrepenynoyl glycero
ls. Triacylglycerols eluted according to the numbers of alkene and alk
yne bonds. Elution times, resolution and quantitation were reproducibl
e over a three-month period. The flame-ionization detector response re
quired no response factors for quantitation of the triacylglycerols pr
esent in Crepis alpina oil. The silver ion chromatography system permi
tted the identification of 95% of the triacylglycerols compared to 70%
of the triacylglycerols previously identified with reversed-phase hig
h-performance liquid chromatography.