ANALYSIS OF RESIDUAL SYNTHETIC ANTIBACTER IALS IN MEAT AND POULTRY MEAT BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

A simple and sensitive HPLC method is described for quantitative deter mination of some synthetic antibacterials in animal tissues. The compo unds are divided into two groups, A and B. Tissue samples are extracte d with acetonitrile and then group A is purified on a silicagel cartri dge and group B by hexane partition; no other clean-up procedure is ne eded. Recoveries of the antibacterials spiked in animal tissue were 64 approximately 99%. The detection limits of quantitative determination by the proposed method were 0.01 ppm for each antibacterial. The cali bration curves for these compounds showed good linearity in the ranges of 0.5 approximately 50 ng for carbadox, 1 approximately 100 ng for t hiamphenicol and furazolidone and 2 approximately 200 ng for other dru gs. Many chicken samples (total 121) which were purchased from a poult ry processing plant in Tokyo were analyzed by the proposed method. Dec oquinate was detected in two samples of chicken muscle at levels of 0. 02 and 0.03 ppm.