SEARCH FOR LT-II AND STB DNA-SEQUENCES AMONG ESCHERICHIA-COLI ISOLATED FROM BOVINE MEAT-PRODUCTS BY COLONY HYBRIDIZATION

Authors
CERQUEIRA AMF TIBANA A GOMES TAT GUTH BEC
Citation
Amf. Cerqueira et al., SEARCH FOR LT-II AND STB DNA-SEQUENCES AMONG ESCHERICHIA-COLI ISOLATED FROM BOVINE MEAT-PRODUCTS BY COLONY HYBRIDIZATION, Journal of food protection, 57(8), 1994, pp. 734-736
Citations number
31
Categorie Soggetti
Food Science & Tenology","Biothechnology & Applied Migrobiology
Journal title
Journal of food protection → ACNP
ISSN journal
0362028X
Volume
57
Issue
8
Year of publication
1994
Pages
734 - 736
Database
ISI
SICI code
0362-028X(1994)57:8<734:SFLASD>2.0.ZU;2-S
Abstract
A total of 1,066 Escherichia call colonies isolated from 105 raw bovin e meat samples purchased at supermarkets in Rio de Janeiro were submit ted to hybridization assays with gene probes for LT-II and STb enterot oxins. Five colonies comprising four different E. call strains isolate d from four pieces of beef, two samples of ground beef (5.7%) and two hamburger patties (5.7%) hybridized with the LT-II probe, while no hyb ridization occurred with the STb probe. Expression of LT-II enterotoxi n using the Y1 adrenal cell assay was verified in two of four E. coil strains. A serotype diversity existed among LT-II E. call strains.