ISOLATION AND CHARACTERIZATION OF POLYGALACTURONASE AND INVERTASE FROM A YEAST ENDOMYCOPSIS-FIBULIGERA-(3812) BIOCONVERSION OF DATE WASTES

Citation
St. Elsayed et al., ISOLATION AND CHARACTERIZATION OF POLYGALACTURONASE AND INVERTASE FROM A YEAST ENDOMYCOPSIS-FIBULIGERA-(3812) BIOCONVERSION OF DATE WASTES, International journal of food science & technology, 29(3), 1994, pp. 255-262
Citations number
24
Categorie Soggetti
Food Science & Tenology
ISSN journal
09505423
Volume
29
Issue
3
Year of publication
1994
Pages
255 - 262
Database
ISI
SICI code
0950-5423(1994)29:3<255:IACOPA>2.0.ZU;2-P
Abstract
Properties of polygalacturonase [E.C. 3.2.1.15] (E(1)) and invertase ( E.C. 3.2.1.26] (E(2)) isolated and purified in powdered form from Endo mycopsis fibuligera grown on date wastes were measured. The molecular weights were estimated to be about 32000 and 69500, and they were high ly specific for hydrolysing (1-4) and (1-2) glycosidic links respectiv ely. The K-m value of E(1) was 7.1 x 10(-2) M for soluble-pectic acid and that for E(2) was 6.7 x 10(-2) M for sucrose at pH4.5, and optimal pHs were 3.5 for E(1) and 4.0 for E(2). The purified enzymes are stab le on storage for more than one year at -20 degrees C.