St. Elsayed et al., ISOLATION AND CHARACTERIZATION OF POLYGALACTURONASE AND INVERTASE FROM A YEAST ENDOMYCOPSIS-FIBULIGERA-(3812) BIOCONVERSION OF DATE WASTES, International journal of food science & technology, 29(3), 1994, pp. 255-262
Properties of polygalacturonase [E.C. 3.2.1.15] (E(1)) and invertase (
E.C. 3.2.1.26] (E(2)) isolated and purified in powdered form from Endo
mycopsis fibuligera grown on date wastes were measured. The molecular
weights were estimated to be about 32000 and 69500, and they were high
ly specific for hydrolysing (1-4) and (1-2) glycosidic links respectiv
ely. The K-m value of E(1) was 7.1 x 10(-2) M for soluble-pectic acid
and that for E(2) was 6.7 x 10(-2) M for sucrose at pH4.5, and optimal
pHs were 3.5 for E(1) and 4.0 for E(2). The purified enzymes are stab
le on storage for more than one year at -20 degrees C.