CHARACTERIZATION OF SUCROSE POLYESTERS TRIACYLGLYCEROLS MIXTURES

High-performance size-exclusion chromatography (HPSEC) and thin-layer chromalography/flame-ionization detection (TLC/FID) have been used for the characterization of mixtures of either monoacid sucrose octaester s with triacylglycerols (TAG) or sucrose polyesters (SPE) prepared fro m oils with natural oils. In mixtures of monoacid su crose octaesters/ TAG, no significant differences were found between the values obtained by either HPSEC or TLC/FID and the actual component proportions. Addi tionally, components could be separated by TLC, which was confirmed by fatty acid composition data of each fraction. Analysis of SPE/oil mix tures was attainable by HPSEC, but alter native quantitation by TLC/FI D required previous silylation. Likewise, fatty acid composition could be determined only in the total mixture and in the sucrose octaester fraction. A formula derived for calculation of oil fatty acid composit ion, based on analytical data, showed the validity of the approach use d in this study to determine component proportions in functional SPE/o il mixtures.