IMS - A NEW SELECTIVE ENRICHMENT TECHNIQUE FOR DETECTION OF SALMONELLA IN FOODS

In a study designed to evaluate the performance of Dynabeads(R) Anti-S almonella, immunomagnetic separation (IMS) followed by plating (IMS-Pl ating) proved far superior to the conventional ISO Salmonella methodol ogy and the Modified Semi-solid Rappaport-Vassiliadis (MSRV) method. S almonella species were isolated and detected from 135 out of the 180 d iverse poultry samples by IMS analysis as against 98 by the convention al method and 33 by the MSRV technique. All results were confirmed bio chemically and serologically. It appeared from the data generated that some of the salmonellae isolated using IMS were greatly inhibited in Rappaport-Vassiliadis enrichment broth at 42-degrees-C and to a lesser extent in selenite cystine broth at 37-degrees-C. A moderately select ive plating medium like XLD proved to be better in isolating these pos sible sensitive wild-type strains of salmonellae than the more selecti ve BGA.