COMPARISON BETWEEN 2 PROCEDURES FOR STEREOSPECIFIC ANALYSIS OF TRIACYLGLYCEROLS FROM VEGETABLE-OILS .1. OLIVE OIL

Two methods for stereospecific analysis of triacylglycerols are compar ed. Procedure A, based on stereospecific phosphorylation of sn-1,2 dia cylglycerols to phosphatidic acids, and procedure B, based on separati on of the diastereomeric 1,2(2,3)-diacylglycerol methane derivatives b y high performance liquid chromatography on silica, were applied to ol ive oil triacyl-sn-glycerols. Statistical evaluation of the results sh owed good reproducibility, and Student's t-test indicates no statistic al differences between the two considered procedures, although some sm all differences were observed and discussed. Fifteen samples of extra- virgin olive oil, produced in the same region (Umbria, Italy), were an alyzed with the two considered procedures.