BEHAVIOR OF DIGLYCERIDES AND CONJUGATED FATTY-ACID TRIGLYCERIDES IN REVERSE-PHASE CHROMATOGRAPHY

The behavior of conjugated fatty acid triglycerides and diglycerides o n reverse-phase chromatography was studied. Trieleostearin is a geomet ric isomer of trilinolenin. The conjugated double bond arrangement in trieleostearin enhances its hydrophobic interaction with the stationar y phase and causes it to be eluted later than trilinolenin. In separat ion of ''critical pairs'' of tri- and diglycerides, diglycerides elute later than triglycerides due to the longer fatty acid constituent. Po sition isomers of 1,2- and 1,3-diglycerides can be separated by revers e-phase high-performance liquid chromatography.