A column chromatography method for separation of phenolic compounds fr
om flaxseed using Sephadex LH-20, RP-8 and silica gel is described. TL
C analysis proved that separated fractions contained numerous phenolic
compounds. All fractions exhibited a maximum UV absorption between 27
0 and 290 nm. Additional shoulders were observed between 274 and 350 n
m. These results imply that lignans and their derivatives are the main
phenolic compounds present in flaxseed.