M. Sosada, OPTIMAL CONDITIONS FOR FRACTIONATION OF RAPESEED LECITHIN WITH ALCOHOLS, Journal of the American Oil Chemists' Society, 70(4), 1993, pp. 405-410
Deoiled rapeseed lecithin was fractionated with ethanol, and optimum c
onditions have been determined to improve purified lecithin yield and
phosphatidylcholine (PC) enrichment. The effect of extraction time, so
lvent volume, ethanol concentration and temperature on the yield and t
he PC enrichment have been described in the form of regression equatio
ns. A full factorial experiment method and a second-order orthogonal d
esign were used in the study. 'rhe regression equations were calculate
d for the maximum value of the response functions optimized by an elec
tronic data processing method, and the results (yield and PC enrichmen
t calculated from regression equations) were compared with those obtai
ned in control experiments. The use of calculated optimal parameters i
n the fractionation process led to 81-96% and 58% increments in yield
and PC enrichment, respectively.