EFFECT OF MEAT CURING AGENTS AND PHOSPHATES ON THIOBARBITURIC ACID (TBA) NUMBERS OF GROUND-BEEF DETERMINED BY THE AQUEOUS ACID-EXTRACTION TBA-C18 METHOD
S. Raharjo et al., EFFECT OF MEAT CURING AGENTS AND PHOSPHATES ON THIOBARBITURIC ACID (TBA) NUMBERS OF GROUND-BEEF DETERMINED BY THE AQUEOUS ACID-EXTRACTION TBA-C18 METHOD, Food chemistry, 47(2), 1993, pp. 137-143
The effects of ascorbic acid (0-0-06%), sodium nitrite (0-0.03%), and
phosphates (0.5%) on thiobarbituric acid (TBA) numbers obtained by a n
ew aqueous acid extraction TBA-C18 method were evaluated both in malon
aldehyde containing aqueous systems and in ground beef samples. Sodium
nitrite significantly (P < 0.05) decreased the formation of red colou
red malonaldehyde TBA complex and addition of sulphanilamide or ascorb
ic acid did not successfully overcome this problem. All the phosphates
tested in this study did not interfere with the formation of the malo
naldehyde TBA complex in both aqueous solutions and ground beef. There
fore, the aqueous acid extraction TBA C18 method can be used for malon
aldehyde measurement in meat products containing phosphates, but inter
ference with the malonaldehyde TBA reaction by nitrite remained a prob
lem.