QUANTITATIVE-ANALYSIS OF SYNTHETIC MIXTURES OF TRIACYLGLYCEROLS WITH FATTY-ACIDS FROM CAPRYLIC TO STEARIC

Separation and quantitation of triacylglycerols tricaprylin to tristea rin was achieved by high-performance liquid chromatography with refrac tive index detection and an eluent composed of propionitrile and butyr onitrile (80-20, vol/vol). Peak identification was based on the logari thms of retention times, and quantitation was achieved by way of theor etical relative response factors. The validity of the response factor calculations was tested by analysis of primary standard mixtures of sa turated triacylglycerols and of triacylglycerols obtained by intereste rification of binary mixtures of monoacid triacylglycerols.