Fatty acid was covalently attached to lipase (EC 3.1.1.3.) from Phycom
yces nites, yielding a modified lipase of higher specific activity in
hydrolytic and synthetic reactions in organic solvents. Attached long-
chain fatty acids solubilized the lipase in organic solvent and, there
fore, promotion of dispersibility in organic solvent resulted in much
higher reactivity. The initial rate of transesterification by modified
lipase was almost 40 times that of native lipase in organic solvent.
The specificities and selectivity of the modified lipase depended on t
he kind of attached fatty acid.