FURTHER OBSERVATIONS ON PROBLEMS ASSOCIATED WITH THE ANALYSIS OF CAROTENOIDS BY HPLC-2 - COLUMN TEMPERATURE

Using a chromatographic system which has previously been shown to give excellent separation of lutein, zeaxanthin, beta-cryptoxanthin, lycop ene and alpha- and beta- carotene, changes in ambient temperature caus ed dramatic differences in the chromatographic response of carotenoids . This study demonstrates the effect of column temperature on the elut ion time and elution profile and the importance of temperature control in reducing analytical variation. The optimum resolution was achieved at 20-22.5-degrees-C.