SPECTROPHOTOMETRIC DETERMINATION OF MANEB, ZINEB AND THEIR DECOMPOSITION PRODUCTS IN SOME VEGETABLES AND ITS APPLICATION TO KINETIC-STUDIESAFTER GREENHOUSE TREATMENT
Mi. Walash et al., SPECTROPHOTOMETRIC DETERMINATION OF MANEB, ZINEB AND THEIR DECOMPOSITION PRODUCTS IN SOME VEGETABLES AND ITS APPLICATION TO KINETIC-STUDIESAFTER GREENHOUSE TREATMENT, Food chemistry, 47(4), 1993, pp. 411-416
A simple and accurate spectrophotometric method has been developed for
the determination of Maneb, Zineb and their decomposition product, et
hylene-thiourea (ETU), using 2,6-dibromoquinone chlorimide (DBQ) and 2
,6-dichloroquinone chlorimide (DCQ). The method depends upon releasing
the ethylenebis-dithiocarbamate (EBDC) moiety from Maneb and Zineb us
ing ethylenediamine tetraacetic acid disodium salt as a solvent. EBDC
then reacts with either DBQ or DCQ yielding a red colour with a maximu
m absorption at 495 nm. ETU, on the other hand produces a yellow colou
r with a maximum absorption at 385 nm. The method was successfully app
lied to the determination of these compounds in cucumber and tomato fr
uits grown in greenhouses. The percentage extraction of Maneb and Zine
b from cucumber was 94.5% and from tomato was 89.2%. The disappearance
of Maneb and Zineb sprayed on cucumber and tomato grown in a greenhou
se followed first-order kinetics. The rates of disappearance of Maneb
and Zineb were correlated to the rate of growth of cucumber and tomato
.