DETERMINATION OF ENROFLOXACIN IN MEAT AND FISH BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

A simple and rapid method was developed for the determination of enrof loxacin (ERFX), a new quinolone antibacterial agent, in fish and meat by high performance liquid chromatography (HPLC) with fluorescence and UV detection. The drug was extracted with 0.2% metaphosphoric acid-ac etonitrile (7 : 3), followed by a Bond Elut C18 clean-up procedure. Th e HPLC separation was carried out on an L-column ODS using 0.05 M sodi um dihydrogenphosphate-acetonitrile (70 : 30) as the mobile phase at a flow rate of 0.5 ml/min. The fluorescence detector was operated at th e excitation wavelength of 285 nm and the emission wavelength of 445 n m, and the ultraviolet detector at 280 nm. The calibration graph was r ectilinear from 1 to 20 ng. The recoveries at the level of 0.4 mug/g w ere 80.2-85.4%, and the detection limit was 0.01 mug/g. An excellent c orrelation was obtained between the results of the HPLC method and the bioassay method (r = 0.99).