PREPARATION, PURIFICATION AND IDENTIFICATION OF 10-OXO-TRANS-8-DECENOIC ACID FROM THE CULTIVATED MUSHROOM, AGARICUS-BISPORUS

A procedure was developed to isolate 10-oxo-trans-8-decenoic acid (ODA ) from mushrooms. ODA was produced by homogenizing mushrooms in phosph ate buffer with added linoleic acid, extracted from the supernatant af ter centrifugation, and purified using column and thin-layer chromatog raphy. The purified compound was then characterized using ultraviolet, infrared and mass spectrometry, and nuclear magnetic resonance spectr oscopy. The purified compound, containing 9 7.5% ODA, was a white, wax y solid with a pK(a) of 4.68. ODA was soluble in acetone, chloroform, ethanol, ethyl ether, methanol, methylene chloride and water, and slig htly soluble in pentane, hexane, heptane and benzene. The TBA test was found to be a viable method for the quantification of ODA.