Lh. Pedersen et al., DETECTION OF PENICILLIUM SPECIES IN COMPLEX FOOD SAMPLES USING THE POLYMERASE CHAIN-REACTION, International journal of food microbiology, 35(2), 1997, pp. 169-177
Rapid identification of filamentous fungi is becoming increasingly imp
ortant in food mycology both for monitoring the production process and
for the identification of food spoilers. This paper describes the dev
elopment and trial of two specific PCR primer sets. A 336 bp fragment
from species belonging to Penicillium subgenus Penicillium was amplifi
ed by the primers ITS 212d and ITS 549. The other primer set, ITS 183
and ITS 401 specifically identified two species, Penicillium roquefort
i and P. carneum, both known as spoilers in the bread industry, by amp
lification of a 300 bp fragment. The future perspectives of PCR based
identification of filamentous fungi in food are discussed. (C) 1997 El
sevier Science B.V.