EFFECTS OF STRESS TREATMENTS ON THE DETECTION OF SALMONELLA-TYPHIMURIUM BY IN-SITU HYBRIDIZATION

In order to assess the usefulness of quantitative in situ rRNA hybridi zation as an indicator of the physiological state of bacteria, we have used this method to measure the cellular contents of 16 S and 23 S rR NA in Salmonella typhimurium subjected to a number of different stress treatments. The contents of rRNA in S. typhimurium decreased when the bacteria were subjected to carbon starvation, heat stress, and osmoti c stress prior to the hybridization, whereas no decrease in the intrac ellular contents of rRNA was observed when the bacteria were subjected to cold stress, acetic acid or ethanol treatment prior to the hybridi zation. We must conclude; that the content of 16 S rRNA and 23 S rRNA cannot be used as the sole indicator of the physiological stale or via bility of food borne pathogens. Viable as well as non-viable food born e bacteria will be detected when methods based on detection of rRNA ar e used. (C) 1997 Elsevier Science B.V.