SPECIES IDENTIFICATIONS IN MARINATED AND HEAT-TREATED MEAT USING ISOELECTRIC-FOCUSING

In order to identify the animal species in marinated and heat-treated marinated meat of bovine (Bos taurus forma domestica), pork (Equus prz ewalskii f. caballus), turkey (Meleagris gallopavo f. domestica), roe deer (Capreolus capreolus capreolus), stag (Cervus elaphus hippelaphus ), springbok (Antidorcas marsupialis), and hare (Lepus europaeus), iso electric focusing followed by silver staining was applied. All samples were marinated under standard conditions. Some samples were also furt her heat treated (45 minutes and 96-degrees-C). In several trials thes e animal species could not be differentiated; neither in the marinated nor in the heat-treated marinated meat samples species-specific prote in bands could be ascertained. By making meat mixtures of two species each (0%, 20%, 40%, 60%, 80%, and 100% parts) protein bands showing an increase of decrease of their intensity could be seen. However, also with this method, a definite differentiation turned out to be impossib le. Nevertheless it is of great importance to dispose of reliable meth ods for the verification of animal species declaration of marinated an d heat-treated marinated meat. Therefore, in consequence to this study , new methods have to be developped.