H. Stutz et al., CAPILLARY ZONE ELECTROPHORETIC DETERMINATION OF HYDROXYMETABOLITES OFATRAZINE IN POTABLE WATER USING SOLID-PHASE EXTRACTION WITH AMBERCHROM RESINS, Mikrochimica acta, 128(1-2), 1998, pp. 107-117
A CZE-separation system for hydroxyatrazine, desisopropylhydroxyatrazi
ne, desethylhydroxyatrazine and ammeline is presented that allows thei
r determination in environmental waters in the sub-mu g L-1 domain aft
er solid-phase extraction/enrichment with styrene-divinylbenzene and m
ethacrylate macroporous (Amberchrom) resins. The CZE separation and de
termination system uses the stacking effect and an increased light pat
h for UV-detection (bubble cell). The etching of the bubble cell was d
one in our laboratory. A ruggedness check of the CZE-system revealed t
he resolution of the N-dealkylated metabolites to be sensitive to pH,
temperature and current variations as well as to changes of capillary
dimensions. Fine-tuning of the separation is performed by variation of
the current intensity in the constant-current mode. Recoveries from f
ortified tap-water samples were 95% for hydroxyatrazine, 70% for deset
hylhydroxyatrazine and approximately 20% for desisopropylhydroxyatrazi
ne at the 0.2 mu g L-1 concentration level. Due to its high polarity,
ammeline is only slightly adsorbed on Amberchrom resins and cannot be
enriched using these materials.