P. Zinin et al., DETERMINATION OF THE DEFOCUSED TRANSFER-FUNCTION OF A CONFOCAL REFLECTION MICROSCOPE BY IMAGING OF A SPHERE, Optik, 107(2), 1997, pp. 45-48
Knowledge of the transfer function of a microscopical system is of imp
ortance for image processing. In this paper three dimensional (3-D) im
ages of spheres from an reflection scanning acoustic microscope are us
ed for the determination of the defocused optical transfer function. T
he presented technique of microscope characterisation can also be appl
ied to confocal optical scanning microscopes.