The concept of the vectorial form of the pupil function in high apertu
re imaging is introduced. Both two-and three-dimensional imaging are c
onsidered. This leads further to the concept of a vectorial optical tr
ansfer function (OTF), which can be used to describe imaging in two or
three dimensions in high aperture systems. In this paper the full vec
torial OTF is calculated for a 0.95 numerical aperture (NA) system and
is shown to be significantly different from the scalar OTF The most s
urprising difference is the occurrence of negative values at certain s
patial frequencies. The fast and accurate calculation of vectorial OTF
for 2-D imaging is achieved using the Fast Fourier transforms found i
n conventional image processing software.