IMMUNOHISTOCHEMICAL OBSERVATIONS IN THE CECA OF CHICKENS INFECTED WITH SALMONELLA-ENTERITIDIS PHAGE TYPE-4

One-day-old specific-pathogen-free White Leghorn chicks were inoculate d orally with Salmonella enteritidis phage type 4 to study adhesion an d invasion of the ceca by immunohistochemistry. Positive staining baci lli were associated with the epithelial surface and were present in th e lumen of the cecal crypts. They were observed in the interstitial ti ssue and in the cytoplasm of macrophage-like cells in the lamina propr ia. A granulomatous nodule containing positive staining bacilli was pr esent in the submucosa of the cecum of one bird at 14 d after inoculat ion.