DETERMINATION OF POLAR COMPOUNDS BY TLC-F ID IN REFINED AND PARTIALLYHYDROGENATED SOJA HEATED OILS

The thermal degradation of oils and fats leads to the formation of pol ar compounds, which is the reason why their determination is adopted f or the quality control of the oil and fat used in flying. Samples of r efined and partially hydrogenated soybean oil were heated for 30 and 6 0 hours respectively, and the polar compounds content were determined using the IUPAC-AOAC official method and TLC-FID. The samples of oil a nd the fractions separated on the column were applied to the chromarod s and developed in petroleum ether: diethyl ether (93:7 v/v). The dete rmination of polar compounds by IUPAC method and by TLC-FID presented similar results (P-value<<0,001), although when the content was greate r than 16% the second method gave higher values. The results showed th at the state of degradation of oils and fats as measured for the quant ity of polar compounds can be determined alternatively by TLC-FID, pre senting a significant reduction in time, hand work and solvent volume.