ATR-FT-IR SPECTROSCOPY OF PROTEINS ADSORBED ON BIOCOMPATIBLE CELLULOSE FILMS

The sorption of the plasma proteins human serum albumin (HSA) and huma n fibrinogen (FIB) onto hemodialytic cellulosic substrates was investi gated by the surface-sensitive ATR-FT-IR spectroscopy. By means of thi s method we monitored quantitatively the kinetics of protein sorption onto acetylated and unmodified cellulose (AKZO-NOBEL), which are used as hemodialytic blood filter material. Furthermore, secondary structur e alterations of the adsorbed proteins were detected and assigned. The spectroscopic findings were compared with the results of zeta potenti al and contact angle measurements in corresponding sorption experiment s and were qualitatively related to blood compatibility.