SIMULTANEOUS DETERMINATION OF GIZZEROSINE AND HISTAMINE IN CHICKEN TISSUES BY HPLC USING AN ON-COLUMN FLUORESCENCE DERIVATIZATION AND COLUMN-SWITCHING TECHNIQUE

A simple and rapid method has been developed for the simultaneous dete rmination of gizzerosine (GZ) and histamine (Him) in chicken tissues b y HPLC using an on-column fluorescence derivatization and column-switc hing technique. GZ and Him in chicken tissues were extracted with perc hloric acid solution, and the crude extract was purified on a Bakerbon d COOH(R) cartridge. GZ and Him were derivatized to the respective o-p hthalaldehyde fluorophore by on-column reaction, followed by column-sw itching and subsequent chromatographic separation on a reversed-phase C-18 polymer column with gradient elution. The calibration curves were rectilinear in the range of 0.02 similar to 1 mu g/mL for GZ, and 0.0 04 similar to 0.2 mu g/mL for Him. The average recoveries were greater than 97% for both GZ and Him added to chicken gizzard at a level of 1 mu g/g.