ADSORPTION OF HETEROCYCLIC AROMATIC-AMINES BY LOW-MOLECULAR-WEIGHT CELLULOSE

This study was carried out to determine the in vitro binding of hetero cyclic aromatic amines (HAAs), IQ, MeIQ, Trp-P-l, and Trp-P-2, to low molecular weight cellulose using HPLC assay and bacterial mutagenicity assay. Eight types of low molecular weight cellulose (degree of polym erization (DP): 125-180, particle size: 8-80 mu m, settling volume in water (SV): 3.2-6.6 mL/g) were used in this study. All types of DP 180 cellulose showed higher adsorptive capacity for IQ, MeIQ, Trp-P-l and Trp-P-2 than the DP 125 and DP 130. The 4 HAAs showed no significant difference in adsorption by cellulose from 10 to 80 mu m in particle s ize. In the case of 2 types of cellulose with the same DP (180) and pa rticle size (40 mu m) but different SV (5.4 mL/g, 4.0 mL/g), Trp-P-l, Trp-P-2 and MeIQ showed no difference in adsorption but IQ was adsorbe d at a higher rate (1.3 times) by the cellulose of larger SV. Highly h ydrophobic compounds among the 4 HAAs (Trp-P-l and Trp-P-2) were adsor bed by cellulose in large quantities, strongly and quickly. The result s of the two assays seemed to show similar patterns in the adsorption of the 4 HAAs by the 8 types of cellulose. Based on the HPLC assay, Tr p-P-l and Trp-P-2 were adsorbed by the 8 types of cellulose at rates o f 81-95% and 71-95%. Similarly, the numbers of revertants per plate in duced by Trp-P-l and Trp-P-2 treated with these celluloses were 0.7-2. 1% and 0.4-5.0% of the control, respectively. From these results, it h as been clarified that low molecular weight cellulose with a DP about 24 to 80 times smaller than the native value can adsorb HAAs.