CHARACTERIZATION OF PECTINATUS-CEREVISIIPHILUS AND P-FRISINGENSIS BY RIBOTYPING

A total of 39 Pectinatus strains have been ribotyped in this investiga tion. Thirteen different ribotypes were observed from 34 strains of P. frisingensis, while three different types were shown with five strain s of P. cerevisiiphilus when digested with EcoRI. These patterns consi sted of two series of fragments: fragments conserved within a species, and polymorphic variation. P. frisingensis had conserved fragments of approximate to 2.53, 7-8, and 10-20 kbp, whereas P. cerevisiiphilus h ad approximate to 2-3, 5-6, and 20-30 kbp. These fragment patterns, ch aracterized by sets of conserved fragments within a species, were spec ies specific. Furthermore, the HindIII- and BamHI-digested restriction patterns have been examined to facilitate differentiation at the stra in level. Thirty-four strains of P. frisingensis could be divided into 1 of 12 types. On the other hand, a composite ribotype, which combine d the ribotypes produced by all restriction enzymes used, produced suc h high definition that all strains of P. frisingensis fell into 1 of 1 7 types.