TYPING OF STAPHYLOCOCCUS-STRAINS BY RANDOM AMPLIFIED POLYMORPHIC DNA ANALYSIS (RAPD) AND BY PULSED-FIELD GEL-ELECTROPHORESIS (PFGE)

Authors
BANNARI S LARPENTGOURGAUD M SIRAMI J MICHAUX O LARPENT JP
Citation
S. Bannari et al., TYPING OF STAPHYLOCOCCUS-STRAINS BY RANDOM AMPLIFIED POLYMORPHIC DNA ANALYSIS (RAPD) AND BY PULSED-FIELD GEL-ELECTROPHORESIS (PFGE), Die Fleischwirtschaft, 78(5), 1998, pp. 502-504
Citations number
5
Categorie Soggetti
Food Science & Tenology
Journal title
Die Fleischwirtschaft → ACNP
ISSN journal
0015363X
Volume
78
Issue
5
Year of publication
1998
Pages
502 - 504
Database
ISI
SICI code
0015-363X(1998)78:5<502:TOSBRA>2.0.ZU;2-4
Abstract
To select potentially aromatic strains of Micrococcaceae, a collection of 16 Staphylococci strains (mainly S. xylosus), isolated from french sausages with a high aromatic potential was typed in accordance to th eir genomes. Two different techniques ware used: The RAPD (Random Ampl ified Polymorphic DNA) and the PFGE (Pulsed Field Gel Electrophoresis) combined with rare cutting endonucleases. In both cases, then genotyp es were obtained. However, a better typing was allowed by the PFGE. Th is method was chosen to achieve the implantation control process of te n strains having a unique profile in dry sausage making.