DETERMINATION OF THE FUNGICIDE TECLOFTALA M AND ITS METABOLITE TECLOFTALAM-IMIDE IN BROWN RICE

The fungicide tecloftalam and its metabolite tecloftalam-imide were in dividually determined. They were extracted from brown rice with aceton e and re-extracted with ethyl acetate. The extract was partitioned bet ween n-hexane and acetonitrile to remove oil, and then charged on a Se p-Pak Plus Florisil cartridge. Tecloftalam-imide was eluted with 50% d iethyl ether-n-hexane and then tecloftalam was eluted with methanol. T ecloftalam-imide was determined on a gas chromatograph (GC) equipped w ith a capillary column DB-1 and an electron capture detector (ECD). Te cloftalam was converted into tecloftalam-imide by addition of acetic a nhydride, and determined by GC-ECD. Recoveries of tecloftalam and tecl oftalam-imide from rice spiked at levels of 0.2 ppm were 89.3 +/- 1.9% and 82.6 +/- 1.5%, respectively. Detection limits were 0.01 ppm in sa mples.