HPLC has been applied for the analysis of neutral phenolic compounds i
n rapeseed. Extraction of phenolic compounds with 70% (v/v) methanol,
separation of neutral and acidic phenolic compounds by solid phase ext
raction with a C-18-cartridge and HPLC separation of the extracts so o
btained are described. Additionally, a rapeseed extract was fractionat
ed by a simple chromatographic method using Sephadex LH-20. Six main c
ompounds from the extract were isolated. A further purification was pe
rformed by preparative HPLC and a C-18-cartridge are used to isolate t
he neutral phenolic compounds. An attempt to identify the compounds wa
s made from the UV-spectra corresponding to each isolated peak of the
chromatogram.