ISOLATION, FRACTIONATION AND HPLC ANALYSIS OF NEUTRAL PHENOLIC-COMPOUNDS IN RAPESEEDS

HPLC has been applied for the analysis of neutral phenolic compounds i n rapeseed. Extraction of phenolic compounds with 70% (v/v) methanol, separation of neutral and acidic phenolic compounds by solid phase ext raction with a C-18-cartridge and HPLC separation of the extracts so o btained are described. Additionally, a rapeseed extract was fractionat ed by a simple chromatographic method using Sephadex LH-20. Six main c ompounds from the extract were isolated. A further purification was pe rformed by preparative HPLC and a C-18-cartridge are used to isolate t he neutral phenolic compounds. An attempt to identify the compounds wa s made from the UV-spectra corresponding to each isolated peak of the chromatogram.