DISTRIBUTION OF DEOXYNIVALENOL IN BARLEY KERNELS INFECTED BY FUSARIUM

Deoxynivalenol (DON) in concentration range 0.1-156.6 mg/kg, was found in 48 samples of barley kernels collected from heads with visible sym ptoms of head scab after inoculation in a field with Fusarium culmorum and Fusarium graminearum. Four fractions of the kernels were collecte d according to their size: >2.8 mm (I); less than or equal to 2.8 - 2. 5 mm (II); >2.5 - 2.2 mm (III) and <2.2 mm (IV). In plants infected wi th Fusarium (when compared to the control) number of kernels in the fr action of the largest kernels (>2.8 mm) decreased from 38% to 18%, whi le in small kernels (fractions <2.5 - 2.2 mm and <2.2 mm) numbers incr eased respectively from 16 to 22% and from 14 to 28%. Average level of DON concentration in these fractions was as follows: (I) 3.5 mg/kg - (4%); (Il) 9.1 mg/kg - (16%); (III) 35.5 mg/kg - (29%) and (IV) 43.3 m g/kg - (51%). The highest DON accumulation was observed in fraction of small (<2.5 mm) kernels. The fraction contributed from 77% up to 94% of total DON content in kernels with low (12 mg/kg) and high (>100 mg/ kg) contamination level, respectively.These results indicate that as w ell level of samples contamination with DON as human and animal health risk in consequence can be reduced by kernels selection (according th eir size) followed by rejection of the smallest kernels fraction.