Ym. Jiang et Jr. Fu, INHIBITION OF POLYPHENOL OXIDASE AND THE BROWNING CONTROL OF LITCHI FRUIT BY GLUTATHIONE AND CITRIC-ACID, Food chemistry, 62(1), 1998, pp. 49-52
Polyphenol oxidase (PPO, EC 1.10.3.2) from litchi peel was partially p
urified by ammonium sulfate fractionation and gel filtration, and a 16
-fold purification of PPO achieved. The use of 10 mmol litre(-1) gluta
thione and 100 mmol litre(-1) citric acid was found to give good contr
ol of the browning of litchi fruit and 80-85% inhibition of PPO observ
ed. Application of glutathione in combination with citric acid is reco
mmended as a way of slowing the browning of litchi fruit. (C) 1998 Els
evier Science Ltd. All rights reserved.