Corn-based food products obtained from commercial outlets in three dif
ferent parts of the U.S., Maryland, Nebraska, and Arizona were analyze
d for total fumonisins by a commercial competitive direct enzyme-linke
d immunosorbant assay (CD-ELISA) and for fumonisin B-1 (FB1) by high-p
erformance liquid chromatography (HPLC). The highest fumonisin concent
rations were found in samples collected in Maryland, where all 18 samp
les were found positive for fumonisins (200 to 7,450 ng/g of food) by
CD-ELISA and 15 of the 18 samples (83%) were found positive for FB1 (<
75 to 5,916 ng/g) by HPLC, Fumonisins were also detected by CD-ELISA i
n 14 of 15 samples collected in Arizona, with concentrations ranging f
rom 200 to 1,450 ng/g, but analyses by HPLC showed that only 8 of 15 s
amples (53%) were positive for FB1(<75 to 1,565 ng/g of food). Of the
23 samples collected in Nebraska, 20 (87%) were positive for fumonisin
s (200 to 2,500 ng/g) by CD-ELISA, but only 10 (44%) were positive for
FB1 (<75 to 927 ng/g) by HPLC. The highest fumonisin arid FB1 concent
rations were found in cornmeal samples, ranging up to 7,450 ng/g of co
rnmeal by CD-ELISA and 5,916 ng/g by HPLC. These findings indicate tha
t there may be a risk of human exposure to fumonisins through the cons
umption of some corn-based foods.