Sr. Kamath et Cv. Morr, TRANSMISSION ELECTRON-MICROSCOPY OF MILKFAT GLOBULES IN THE LOW-DENSITY LIPID FRACTION OF SWISS CHEESE WHEY, Journal of the American Oil Chemists' Society, 75(6), 1998, pp. 745-747
A low-density lipid fraction (LDLF) was recovered from Swiss cheese wh
ey (SCW) residual lipids by highspeed centrifugation. The present stud
y was conducted to determine the microstructural properties of the ext
remely small-sized milkfat globules (MFG) in LDLF by: (i) freeze-fract
ure transmission electron microscopy (FF-TEM) and (ii) thin-section TE
M (TS-TEM). FF-TEM results revealed that MFG in LDLF were less than or
equal to 1 mu m in size and were embedded in what appeared to be a sm
ooth, protein-like matrix. The MFG in FF-TEM specimens exhibited eithe
r planarly cleaved fractions with smooth cores or peripherally cleaved
fractions with surface laminations. TS-TEM results revealed that the
MFG in LDLF were dispersed in an aggregated nonlipid matrix.